Before the experiment, the sterile room and the sterile console are irradiated with ultraviolet lamp for 30-60 minutes for sterilization, the sterile operation lifting surface is wiped with 70% alcohol, and the fan of the sterile console is turned on for 10 minutes before the experimental operation is started.
Take sterile test articles carefully to avoid contamination. Do not touch the tip of the pipette or the mouth of the container, and do not operate the experiment directly above the open container. After the container is opened, clamp the bottle cap and hold the bottle body by hand, tilt it at an angle of about 45 °, and try not to place the opening of the bottle cap upward on the table.
Staff should pay attention to their own safety and carry out the experiment only after wearing experimental clothes and gloves. For human or virus infected cell lines, special care should be taken and an appropriate level of sterile console (at least class II) should be selected. During operation, avoid the generation of aerosol substances, be careful of toxic drugs, such as DMSO and TPA, and avoid the injury of sharp needles.
Before and after each use, the inoculation needle must be burned and sterilized by flame. After cooling, the culture can be inoculated.
The sterile operation work area shall be kept clean and spacious. Necessary items, such as test tube rack, pipette aspirator or pipette box, can be placed temporarily. Other experimental supplies shall be removed after use to facilitate the flow of air. The experimental articles are wiped with 70% alcohol before being brought into the sterile operation table. The experimental operation should be carried out in the sterile area in the center of the lifting surface, not in the non sterile area at the edge.
When using the solution in the sterile bottle, do not block the bottle mouth with sterile dressing, pour the sterile solution, or directly dip it into the solution bottle to avoid polluting the remaining solution.
During each operation, a negative control shall be made to check the reliability of aseptic operation.
If bacterial liquid is spilled on the table or the ground, it shall be immediately overturned at the contaminated place with 5% carbolic acid solution or 3% Lysol for at least 30 minutes before treatment. When the working clothes and hats are contaminated by bacterial solution, they shall be removed immediately and washed after high-pressure steam sterilization.
If the articles in the sterile bag are accidentally polluted or the sterile bag is soaked, external microorganisms can penetrate into the bag, causing pollution, which needs to be disinfected again.
All articles with live bacteria must be disinfected before they can be washed under the faucet. It is strictly prohibited to pollute the sewer.
When sucking bacterial liquid, you must use an ear ball to suck it. Do not directly touch the straw with your mouth.
Straws, test tubes, Petri dishes and other utensils with bacterial solution shall be soaked in a disinfection barrel containing 5% Lysol solution for disinfection, and taken out for flushing after 24 hours.
When wearing gloves, it should be noted that the hand without gloves cannot touch the outside of the glove, while the hand with gloves cannot touch the hand without gloves or the inside of another glove. If the gloves are found broken after wearing, they should be replaced immediately. When taking off the glove cover, turn the glove mouth under the glue, and do not force the edge or finger of the glove to avoid damage.
After the experiment, take the experimental articles out of the workbench and wipe the sterile operation surface with 70% alcohol. The operation interval shall be after the sterile operation table has operated for more than 10 minutes.
In general, the laboratory decoration is not an ordinary tooling decoration, but has its own specific standards and specifications. There are many details to pay attention to in the design and decoration of the sterile room. In the decoration, we must comprehensively consider the overall planning and reasonable layout of the laboratory. At present, most aseptic rooms exist in microbial factories, and ultra clean tables are used in general laboratories. The main function of the super clean table is to use the air laminar flow device to remove all kinds of micro dust including microorganisms on the upper part of the worktable. Through the electric device, the air enters the worktable after passing through the high-efficiency filter, so that the worktable is always under the control of flowing sterile air. Moreover, there is a high-speed air curtain on the side close to the outside to prevent the entry of external bacteria carrying air. With the improvement of laboratory decoration level, laboratory staff have higher and higher requirements for design,
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